John Morris

Advanced, intelligent features enable better, faster purification
The Biotage Isolera flash purification family is designed to help purify synthesis reaction mixtures, natural product extracts, and other mixes of organic compounds requiring separation and purification. Two systems are available in several configurations providing purification of milligrams to >150g.

Biotage Isolera Spektra is available in a single cartridge base model, the Biotage Isolera Spektra One, and a 4-cartridge configuration, the Biotage Isolera Spektra Four, which is ideal for multi-user or high-throughput laboratories.

Achieve up to 30% solvent savings with Gradient Optimisation "GO"
Step-gradients can be short and powerful providing separations for one or more compounds but can be challenging to develop and optimise. Isolera Spektra provides gradient optimisation through new, patent-pending TLC-to-Step Gradient technology. Using solvent and TLC Rf values, Isolera Spektra builds a gradient to separate all the compounds (up to 6) in the sample. This new technology will also provide cartridge selection guidance based on the cartridge loading capacity and purification speed. The step gradient can also be used to isolate a targeted compound reducing run time and solvent use further.

Figure 1. Using a TLC-to-Step gradient simulation, a 3-component sample was purified using a Biotage® SNAP HP-Sil 10g cartridge in less than 9 CV. At a flow rate of 12 ml/min the actual run time was about 9 minutes and used 130ml of solvent.

Improve fraction and compound purity with ?-All detection and PDA spectral analysis
New PDA scanning and ?-All technology detects any UV absorbing compound eluting from a flash cartridge while measuring and displaying each eluting compound's individual UV spectrum. Combined with novel baseline rise correction, yield losses to improper wavelength selection and large fraction volumes are no longer a concern. The PDA spectra can be reviewed and used to determine fraction purity eliminating the need for post-flash purity analysis.

Figure 2. All spectra are stored and are reviewable in the Results tab. The 2D data can be used to verify peak UV maxima and also peak purity just by moving a cursor over the peak or area of interest. If the spectrum remains consistent during the scan the fraction is pure. The purity data eliminates using TLC to determine which fractions contain pure compound and which tubes can be combined, which can save up to 1.5 hours a day in fraction analysis.

Elute complex samples with the Quatro-binary gradient
Use up to four solvents in a single gradient to easily purify samples with diverse polarity. With Quatro-binary gradient capability traditional binary gradients with a limited polarity range can be adjusted to elute very lipophilic and highly polar compounds within a single purification. Or compound solubility during the separation by adding a constant amount of a co-solvent, acid, or base with the Isolera's advanced pump.

Maintain compound solubility with a ternary co-solvent
Isocratically pump a third solvent into any binary gradient to help maintain compound solubility eliminating potential over-pressure issues caused by precipitating compounds.

Enhance productivity with on-the-fly editing
Biotage Isolera methods can easily be edited either in front of the Isolera or from the comfort of your office. Edit the gradient (click & drag points AND segments), flow rate, collection volume, fraction wavelengths and modes, and add more collection racks if you need to - all while the run is in progress. Gradient changes can now be made with a simplified graphical interface or through a table layout.

Achieve superior purification with Biotage flash cartridges
The Biotage Isolera Spektra One and Isolera Spektra Four systems can use various styles including Biotage SNAP and Biotage® SNAP Ultra with silica masses from 5g to 750g.

Key Features

• TLC-to-Step gradient
• Gradient Optimisation
• Real-time PDA scanning
• 2D and 3D spectral analysis
• Optimised for Biotage® SNAP Ultra cartridges
• ?-All detection
• Baseline correction
• Run counter

Additional Features

• Largest integrated fraction capacity
• Quatro-binary Gradient - use up to four solvents in a single gradient
• Isocratic co-solvent - increase compound solubility and recovery
• Default Methods - simplify method creation
• On-the-fly editing - modify purification runs in progress
• Biotage SNAP cartridges - increase loading capacity and improve compound separations.
• Add an auxiliary detector - use other detectors such as the Biotage Isolera ELSD-1080
• An assortment of Biotage SNAP cartridges is included with the Isolera system

Now even more flexibility and capability
Need to hold a gradient to improve a separation?
It is now possible with a new Isocratic Hold button.

Need to collect a large volume of a single eluting compound?
A new fraction collector bypass feature provides collection of any volume in any clean collection vessel, saving fraction capacity.

Need to multi-task?
Monitor the purification from your office and you can advance fractions, use the Isocratic hold feature, monitor solvent and waste levels, pause the purification run, just to name a few.

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Biotage® Isolera™ Spektra technical note

Flash Purification Systems brochure

Isolera User's Manual

Application Note: Flash chromatographic isolation and characterisation of organic molecules using advanced ?-All detection with full range photo-diode array analysis

Application Note: Flash chromatography with diode array scanning rapidly confirms fraction purity

Application Note: Using Acetone in UV-based Gradient Flash Chromatography